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PrimeFlow™


PrimeFlow™ NEWS

UPDATE 9/11/2016 - Innovations to the PrimeFlow™ Technology

PrimeFlow™ is now capable of 4 colour multiplexing, can be used to detect miRNA and compensation controls are now included in kits.

PrimeFlow™ RNA additional resources 
 

 WEBINARS: 

 HIV Persistence 

Daniel Kaufmann, PhD
Clinical Professor, Department of Medicine
University of Montreal and CHUM Research Center

REGISTER - on demand

  

 

Single-cell analysis of virus infection: Zika and beyond?

 Eric Clambey, PhD
Assistant Professor, Department of Anesthesiology and Program in Immunology
University of Colorado 

 WATCH - YouTube

 

High throughput detection of gene-specific mRNA at the single cell level by flow cytometry

Filippos Porichis, PhD
Director of International Programs at the Ragon Institute, Massachusetts General Hospital (MGH), MIT, and Harvard

WATCH - YouTube

 

 


PrimeFlow™

eBioscience and Jomar Life Research are excited to announce the Australian/NZ launch of PrimeFlow™, a ground-breaking new technology that, for the first time, allows the detection of RNA targets in single cells by flow cytometry! 
 
PrimeFlow opens the door to many new avenues of research which were previously inaccessible in single cells: Understand gene regulation by comparing RNA and protein expression in the same cell; Study targets for which no antibody-based probe exists; Understand the role of non-coding RNAs.  PrimeFlow is fully compatible with most current labels and protocols for flow cytometry. 
 
The term “Revolutionary” is often used lightly, but we believe you will agree that PrimeFlow truly fits this description as a ground-breaking new flow cytometry technology!

  • Detect RNA and protein simultaneously
  • See gene expression hetergenity at the single-cell level
  • Compare RNA and protein kinetics in the same cell
  • Detect non-coding RNA in cellular subsets
  • Evaluate viral RNA in infected cells
  • Analyze mRNA expression levels when antibody selection is limited

To learn more about PrimeFlow™ technology and how it can enable your research, download the PrimeFlow™ White Paper, the PrimeFlow™ Brochure or a Scientific Spotlight article explaining the origin of the technology from Dr. Filippos Porichis at The Ragon Institute of MGH, MIT and Harvard.

Want to be contacted by a PrimeFlow™ technology expert? Click here

 

PrimeFlow™ RNA Assay Technology White Paper | PDF online  | PrimeFlow™ Website

Introduction 


PrimeFlow™ RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cells change over time or in response to stimulation. This novel assay uses fluorescent in situ hybridization (FISH) to enable simultaneous detection of as many as three RNA transcripts in a single cell using a standard flow cytometer. PrimeFlow RNA Assay is compatible with cell surface and intracellular staining, using common flow cytometry fluorochromes. The assay is based upon proven and well-published ViewRNA assays designed for microscopic analysis of RNA in cells and tissues that combine paired oligonucleotide probe design with branched DNA (bDNA) signal amplification to robustly detect gene expression at the single-cell level.

Full version PDF