Serving Australia and NZ
1300 543 373
Return to Previous Page

Human Caspase-9 Platinum ELISA 10 x 96 tests

Human Caspase-9 Platinum ELISA 10 x 96 tests

Quick Overview:

Human Caspase-9 Platinum ELISA 10 x 96 tests

Product Code: BMS2025TEN

  • Details

    The human Caspase-9 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-9. The human Caspase-9 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

    Caspases are the executioners of apoptosis. This cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis.

    Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP (3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP.
    Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its subcellular redistribution and activation. Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and ?7 are targets of caspase-9 activity. Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a so called apoptosome, amplifying the caspase cascade.

    Feedbackcleavage of caspase-9 by caspase-3 again significantly increases the activity of the apoptosome. Caspase-9 plays a crucial role in all pathologies involving apoptotic processes. Specifically the involvement of caspase-9 has been described in human gastric cancer, ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimer?s disease, Huntington?s disease and osteoarthritis.
  • Additional Information

    SKU BMS2025TEN
    Name Human Caspase-9 Platinum ELISA 10 x 96 tests
    Size 10 x 96 tests
    Product Type Platinum ELISAs
    Datasheet View Datasheet
    Supplier eBioscience
    Applications ELISA
    Species reactivity (expected) Human
    General Readings Renatus,M.; Stennicke,H.R.; Scott,F.L.; Liddington,R.C.; Salvesen,G.S.. Dimer formation drives the activation of the cell death protease caspase 9. Proc.Natl.Acad.Sci.U S A 2001;98:14250-14255. (Link)

    Susin,S.A.; Lorenzo,H.K.; Zamzami,N.; Marzo,I.; Brenner,C.; Larochette,N.; Prevost,M.C.; Alzari,P.M.; Kroemer,G.. Mitochondrial release of caspase-2 and -9 during the apoptotic process. J.Exp.Med. 1999;189:381-394. (Link)

    Kiechle,T.; Dedeoglu,A.; Kubilus,J.; Kowall,N.W.; Beal,M.F.; Friedlander,R.M.; Hersch,S.M.; Ferrante,R.J.. Cytochrome C and caspase-9 expression in Huntington's disease. Neuromolecular.Med. 2002;1:183-195. (Link)

    Li,P.; Nijhawan,D.; Budihardjo,I.; Srinivasula,S.M.; Ahmad,M.; Alnemri,E.S.; Wang,X.. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-489. (Link)

    Kuida,K.. Caspase-9 72. Int.J.Biochem.Cell Biol. 2000;32:121-124. (Link)

    Johnson,C.R.; Jarvis,W.D.. Caspase-9 regulation: an update. Apoptosis 2004;9:423-427. (Link)

    Rodriguez,J.; Lazebnik,Y.. Caspase-9 and APAF-1 form an active holoenzyme 106. Genes Dev. 1999;13:3179-3184. (Link)

    McNeish,I.A.; Bell,S.; McKay,T.; Tenev,T.; Marani,M.; Lemoine,N.R.. Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway. Exp.Cell Res. 2003;286:186-198. (Link)

    Catchpoole,D.R.; Lock,R.B.. The potential tumour suppressor role for caspase-9 (CASP9) in the childhood malignancy, neuroblastoma. Eur.J.Cancer 2001;37:2217-2221. (Link)

    Teitz,T.; Wei,T.; Liu,D.; Valentine,V.; Valentine,M.; Grenet,J.; Lahti,J.M.; Kidd,V.J.. Caspase-9 and Apaf-1 are expressed and functionally active in human neuroblastoma tumor cell lines with 1p36 LOH and amplified MYCN. Oncogene 2002;21:1848-1858. (Link)

    Matsuo,M.; Nishida,K.; Yoshida,A.; Murakami,T.; Inoue,H.. Expression of caspase-3 and -9 relevant to cartilage destruction and chondrocyte apoptosis in human osteoarthritic cartilage. Acta Med.Okayama 2001;55:333-340. (Link)

    Marsden,V.S.; O'Connor,L.; O'Reilly,L.A.; Silke,J.; Metcalf,D.; Ekert,P.G.; Huang,D.C.; Cecconi,F.; Kuida,K.; Tomaselli,K.J.; Roy,S.; Nicholson,D.W.; Vaux,D.L.; Bouillet,P.; Adams,J.M.; Strasser,A.. Apoptosis initiated by Bcl-2-regulated caspase activation independently of the cytochrome c/Apaf-1/caspase-9 apoptosome. Nature 2002;419:634-637. (Link)

    Izawa,M.; Mori,T.; Satoh,T.; Teramachi,K.; Sairenji,T.. Identification of an alternative form of caspase-9 in human gastric cancer cell lines: a role of a caspase-9 variant in apoptosis resistance. Apoptosis. 1999;4:321-325. (Link)

    Shinoura,N.; Sakurai,S.; Shibasaki,F.; Asai,A.; Kirino,T.; Hamada,H.. Co-transduction of Apaf-1 and caspase-9 highly enhances p53-mediated apoptosis in gliomas 110. Br.J.Cancer 2002;86:587-595. (Link)

    Zou,H.; Yang,R.; Hao,J.; Wang,J.; Sun,C.; Fesik,S.W.; Wu,J.C.; Tomaselli,K.J.; Armstrong,R.C.. Regulation of the Apaf-1/caspase-9 apoptosome by caspase-3 and XIAP. J.Biol.Chem. 2003;278:8091-8098. (Link)

    Rohn,T.T.; Rissman,R.A.; Davis,M.C.; Kim,Y.E.; Cotman,C.W.; Head,E.. Caspase-9 activation and caspase cleavage of tau in the Alzheimer's disease brain. Neurobiol.Dis. 2002;11:341-354. (Link)
    Storage +4C