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Human sPSGL-1 Platinum ELISA 96 tests

Human sPSGL-1 Platinum ELISA 96 tests

Quick Overview:

Human sPSGL-1 Platinum ELISA 96 tests

Product Code: BMS255

  • Details

    The human PSGL-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human PSGL-1. The human PSGL-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

    Selectins are part of the broader family of cell adhesion molecules. The family of selectins consists of three structurally and functionally related molecules, L-selectin, P-selectin and E-selectin.

    P-selectin glycoprotein ligand-1 is found on white blood cells and endothelial cells and plays an important role in the recruitment of white blood cells into inflamed tissue. PSGL-1 binds with the highest affinity to P-selectin.
  • Additional Information

    SKU BMS255
    Name Human sPSGL-1 Platinum ELISA 96 tests
    Size 96 tests
    Product Type Platinum ELISAs
    Datasheet View Datasheet
    Supplier eBioscience
    Applications ELISA
    Species reactivity (expected) Human
    General Readings Li,F.; Erickson,H.P.; James,J.A.; Moore,K.L.; Cummings,R.D.; McEver,R.P.. Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies. J.Biol.Chem. 1996;271:6342-6348. (Link)

    Goetz,D.J.; Ding,H.; Atkinson,W.J.; Vachino,G.; Camphausen,R.T.; Cumming,D.A.; Luscinskas,F.W.. A human colon carcinoma cell line exhibits adhesive interactions with P-selectin under fluid flow via a PSGL-1-independent mechanism 45. Am.J.Pathol. 1996;149:1661-1673. (Link)

    Sako,D.; Comess,K.M.; Barone,K.M.; Camphausen,R.T.; Cumming,D.A.; Shaw,G.D.. A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding. Cell 1995;83:323-331. (Link)

    Laszik,Z.; Jansen,P.J.; Cummings,R.D.; Tedder,T.F.; McEver,R.P.; Moore,K.L.. P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells 76. Blood 1996;88:3010-3021. (Link)

    McEver,R.P.. Regulation of function and expression of P-selectin. Agents Actions Suppl 1995;47:117-119. (Link)

    Hirose,M.; Kawashima,H.; Miyasaka,M.. A functional epitope on P-selectin that supports binding of P-selectin to P-selectin glycoprotein ligand-1 but not to sialyl Lewis X oligosaccharides. Int.Immunol. 1998;10:639-649. (Link)

    Patel,K.D.; McEver,R.P.. Comparison of tethering and rolling of eosinophils and neutrophils through selectins and P-selectin glycoprotein ligand-1. J.Immunol. 1997;159:4555-4565. (Link)

    Borges,E.; Tietz,W.; Steegmaier,M.; Moll,T.; Hallmann,R.; Hamann,A.; Vestweber,D.. P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin. J.Exp.Med. 1997;185:573-578. (Link)

    Pouyani,T.; Seed,B.. PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus. Cell 1995;83:333-343. (Link)

    Hidari,K.I.; Weyrich,A.S.; Zimmerman,G.A.; McEver,R.P.. Engagement of P-selectin glycoprotein ligand-1 enhances tyrosine phosphorylation and activates mitogen-activated protein kinases in human neutrophils. J.Biol.Chem. 1997;272:28750-28756. (Link)

    Snapp,K.R.; Craig,R.; Herron,M.; Nelson,R.D.; Stoolman,L.M.; Kansas,G.S.. Dimerization of P-selectin glycoprotein ligand-1 (PSGL-1) required for optimal recognition of P-selectin. J.Cell Biol. 1998;142:263-270. (Link)

    Symon,F.A.; Lawrence,M.B.; Williamson,M.L.; Walsh,G.M.; Watson,S.R.; Wardlaw,A.J.. Functional and structural characterization of the eosinophil P-selectin ligand. J.Immunol. 1996;157:1711-1719. (Link)

    Sako,D.; Chang,X.J.; Barone,K.M.; Vachino,G.; White,H.M.; Shaw,G.; Veldman,G.M.; Bean,K.M.; Ahern,T.J.; Furie,B.; .. Expression cloning of a functional glycoprotein ligand for P-selectin. Cell 1993;75:1179-1186. (Link)

    Mehta,P.; Patel,K.D.; Laue,T.M.; Erickson,H.P.; McEver,R.P.. Soluble monomeric P-selectin containing only the lectin and epidermal growth factor domains binds to P-selectin glycoprotein ligand-1 on leukocytes. Blood 1997;90:2381-2389. (Link)

    Vachino,G.; Chang,X.J.; Veldman,G.M.; Kumar,R.; Sako,D.; Fouser,L.A.; Berndt,M.C.; Cumming,D.A.. P-selectin glycoprotein ligand-1 is the major counter-receptor for P-selectin on stimulated T cells and is widely distributed in non-functional form on many lymphocytic cells. J.Biol.Chem. 1995;270:21966-21974. (Link)

    Borges,E.; Pendl,G.; Eytner,R.; Steegmaier,M.; Zollner,O.; Vestweber,D.. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J.Biol.Chem. 1997;272:28786-28792. (Link)

    Snapp,K.R.; Ding,H.; Atkins,K.; Warnke,R.; Luscinskas,F.W.; Kansas,G.S.. A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin. Blood 1998;91:154-164. (Link)

    Goetz,D.J.; Greif,D.M.; Ding,H.; Camphausen,R.T.; Howes,S.; Comess,K.M.; Snapp,K.R.; Kansas,G.S.; Luscinskas,F.W.. Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin. J.Cell Biol. 1997;137:509-519. (Link)

    McEver,R.P.; Cummings,R.D.. Role of PSGL-1 binding to selectins in leukocyte recruitment. J.Clin.Invest 1997;100:S97-103. (Link)

    McEver,R.P.; Cummings,R.D.. Perspectives series: cell adhesion in vascular biology. Role of PSGL-1 binding to selectins in leukocyte recruitment. J.Clin.Invest 1997;100:485-491. (Link)

    Dulkanchainun,T.S.; Goss,J.A.; Imagawa,D.K.; Shaw,G.D.; Anselmo,D.M.; Kaldas,F.; Wang,T.; Zhao,D.; Busuttil,A.A.; Kato,H.; Murray,N.G.; Kupiec-Weglinski,J.W.; Busuttil,R.W.. Reduction of hepatic ischemia/reperfusion injury by a soluble P-selectin glycoprotein ligand-1. Ann.Surg. 1998;227:832-840. (Link)

    Tannapfel,A.; Kohlhaw,K.; Ebelt,J.; Hauss,J.; Liebert,U.; Berr,F.; Wittekind,C.. Apoptosis and the expression of Fas and Fas ligand (FasL) antigen in rejection and reinfection in liver allograft specimens. Transplantation 1999;67:1079-1083. (Link)

    Kaytes,P.S.; Geng,J.G.. P-selectin mediates adhesion of the human melanoma cell line NKI-4: identification of glycoprotein ligands. Biochemistry 1998;37:10514-10521. (Link)

    Liu,W.; Ramachandran,V.; Kang,J.; Kishimoto,T.K.; Cummings,R.D.; McEver,R.P.. Identification of N-terminal residues on P-selectin glycoprotein ligand-1 required for binding to P-selectin. J.Biol.Chem. 1998;273:7078-7087. (Link)

    Blanks,J.E.; Moll,T.; Eytner,R.; Vestweber,D.. Stimulation of P-selectin glycoprotein ligand-1 on mouse neutrophils activates beta 2-integrin mediated cell attachment to ICAM-1. Eur.J.Immunol. 1998;28:433-443. (Link)
    Storage +4C